Abstract
Ischemic heart diseases (IHDs) cause great morbidity and mortality worldwide, necessitating effective treatment. Salvianic acid A sodium (SAAS) is an active compound derived from the well-known herbal medicine Danshen, which has been widely used for clinical treatment of cardiovascular diseases in China. This study aimed to confirm the cardioprotective effects of SAAS in rats with myocardial infarction and to investigate the underlying molecular mechanisms based on proteome and transcriptome profiling of myocardial tissue. The results showed that SAAS effectively protected against myocardial injury and improved cardiac function. The differentially expressed proteins and genes included important structural molecules, receptors, transcription factors, and cofactors. Functional enrichment analysis indicated that SAAS participated in the regulation of actin cytoskeleton, phagosome, focal adhesion, tight junction, apoptosis, MAPK signaling, and Wnt signaling pathways, which are closely related to cardiovascular diseases. SAAS may exert its cardioprotective effect by targeting multiple pathways at both the proteome and transcriptome levels. This study has provided not only new insights into the pathogenesis of myocardial infarction but also a road map of the cardioprotective molecular mechanisms of SAAS, which may provide pharmacological evidence to aid in its clinical application.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (Grant Nos. 81503039, 81573396, and 81773683) and the Discipline Leader Training Program of Pudong New Area Health System of Shanghai (PWRd2014-11). D.J. would like to give special thanks to the BGI-Shenzhen Corporation for its help in the experiments.
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CZ, JH, ZYZ, CZZ, YQ, and DJ designed the experiments; DJ, CZZ, YQ, and XFC performed the experiments and analyzed the data; DJ, YQ, and LJ wrote the manuscript; and CZ, JH, ZYZ, YFC, and AFC reviewed the manuscript.
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Jia, D., Zhang, Cz., Qiu, Y. et al. Cardioprotective mechanisms of salvianic acid A sodium in rats with myocardial infarction based on proteome and transcriptome analysis. Acta Pharmacol Sin 40, 1513–1522 (2019). https://doi.org/10.1038/s41401-019-0265-1
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DOI: https://doi.org/10.1038/s41401-019-0265-1
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