Fig. 1: The structure and properties of the anti-HER2×PD1 BsAb. | Acta Pharmacologica Sinica

Fig. 1: The structure and properties of the anti-HER2×PD1 BsAb.

From: Bispecific antibody simultaneously targeting PD1 and HER2 inhibits tumor growth via direct tumor cell killing in combination with PD1/PDL1 blockade and HER2 inhibition

Fig. 1

a Schematics of the anti-HER2×PD1 BsAb structure. b SDS-PAGE showing nonreduced and reduced anti-HER2×PD1 BsAb. Lane 1: nonreduced BsAb; Lane 2: reduced BsAb; Lane 3: nonreduced trastuzumab; Lane 4: reduced trastuzumab; M: Molecular weight markers. c SEC chromatogram showing that the BsAb purified by a single-step protein A affinity column had over 95% monomeric species. d Differential scanning calorimetry (DSC) of the anti-HER2×PD1 BsAb showing that the antibody has a Tonset (the temperature at onset of melting) of 52.5 °C and Tm1/2/3 (melting temperatures) of 59.2 °C/68.4 °C/ 83.5 °C, respectively.

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