Fig. 6: DHC limits p65 and ERK activation in BMDMs. | Acta Pharmacologica Sinica

Fig. 6: DHC limits p65 and ERK activation in BMDMs.

From: Antiatherosclerotic effect of dehydrocorydaline on ApoE−/− mice: inhibition of macrophage inflammation

Fig. 6

a BMDMs were treated with 100 ng/mL LPS for 0, 15, or 30 min and incubated with DMSO or 100 μM DHC. n = 8. b BMDMs were treated with 0, 50, and 100 ng/mL LPS for 15 min and incubated with DMSO or 100 μM DHC. n = 5. a, b Immunoblots showing p-p65, p65, p-ERK1/2, ERK1/2, p-p38, p38, p-JNK, JNK, and GAPDH in the cells. c Immunofluorescent staining of p65 in BMDMs treated with DMSO or 100 μM DHC in the presence or absence of 100 ng/mL LPS for 15 min. n = 4. Scale bar, 50 μm. The data are shown as the mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001 vs. as indicated.

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