Fig. 2: PPX administration attenuated astrocyte activation and IL-1β generation in the striatum of LPS-injected mice. | Acta Pharmacologica Sinica

Fig. 2: PPX administration attenuated astrocyte activation and IL-1β generation in the striatum of LPS-injected mice.

From: Pramipexole inhibits astrocytic NLRP3 inflammasome activation via Drd3-dependent autophagy in a mouse model of Parkinson’s disease

Fig. 2

a–c ELISA results for TNF-α (a, n = 5), IL-1β (b, n = 6), and IL-10 (c, n = 5) levels in the striatum of mice. d–h Immunofluorescent anti-GFAP staining for studying astrocyte activation/proliferation in the striatum. GFAP+ cells quantification shown in e, n = 4–6 mice per group. The lower panel in d showed a representative GFAP+ cell morphology generated by Sholl analysis, and group data for ending radius (f), summarized intersects (g) and ramification index (h) are shown in f–h, n = 20 cells per group for analysis. The interval of the concentric circles is 10 μm. Scale bar: 100 μm (upper panel) and 20 μm (lower panel). i–m Immunofluorescent staining with anti-Iba1 to assess microglia activation in the striatum and microglia morphological changes by Sholl analysis (n = 20 cells per group), with 5 μm intervals for the concentric circles. Iba1+ cells quantification results are shown in j–m. One-way ANOVA followed by Tukey’s post hoc analysis. Mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. as indicated; ns, not significant.

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