Fig. 2: Her induces autophagy in PDAC cells. | Acta Pharmacologica Sinica

Fig. 2: Her induces autophagy in PDAC cells.

From: Hernandezine induces autophagic cell death in human pancreatic cancer cells via activation of the ROS/AMPK signaling pathway

Fig. 2

a, b Capan-1 and SW1990 cells were treated with various concentrations of Her (0–40 μM) for 24 h. Western blot analysis of LC3-I, LC3-II and SQSTM1/p62 levels in Capan-1 and SW1990 cells. c, d Capan-1 and SW1990 cells were treated with Her (10 μM) for 2 h, 4 h, 6 h, 12 h and 24 h, and Western blot analysis of LC3-I and LC3-II levels was performed. e, f Capan-1 and SW1990 cells were treated with Her (10 μM) for 24 h, and autophagic vacuoles (red arrow) in cells were observed by TEM. Data are presented as the mean ± SD, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the control group.

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