Fig. 1: MANF is upregulated in inflammatory colon tissues.

a Representative immunohistochemical staining of paraffin-embedded sections of colon specimens obtained from patients with UC (n = 31), CD (n = 13), and healthy controls (n = 11). Scale bars: 20 μm (original insets) and 10 μm (enlarged insets). b The quantitative date in panel a. c MANF mRNA levels in colon tissues of control mice and the mice treated with DSS were detected by using qPCR. d MANF protein levels in colon tissues of control mice and the mice treated with DSS were detected by Western blot. e The quantitative data in panel d. f MANF protein levels in colon tissues of control mice and the mice treated with DSS were detected by immunohistochemistry assay. g The quantitative data in panel f. All data are expressed as the mean ± SEM from 6 mice. *P < 0.05, **P < 0.01, ***P < 0.001, compared with normal controls.