Fig. 2: MANF is upregulated in the macrophages of colitis tissues.

a Immunofluorescence staining for CD68 (red) and MANF (green) in human colon tissues. DAPI (blue) was used to stain nuclei. Scale bars: 50 μm (original insets) and 30 μm (enlarged insets). b Immunofluorescence staining for MANF (red) and CD68 (green) in mice colon tissues. DAPI (blue) was used to stain nuclei. Scale bars: 50 μm (original insets) and 30 μm (enlarged insets). c Immunofluorescence staining for MANF (green) and CD68 (red) in peritoneal macrophages isolated from mice. DAPI (blue) was used to stain nuclei. Scale bars: 10 μm.