Fig. 7: BoNT/A treatment alleviated synapse and spine loss in the reserpine-treated mouse hippocampus. | Acta Pharmacologica Sinica

Fig. 7: BoNT/A treatment alleviated synapse and spine loss in the reserpine-treated mouse hippocampus.

From: Botulinum neurotoxin A ameliorates depressive-like behavior in a reserpine-induced Parkinson’s disease mouse model via suppressing hippocampal microglial engulfment and neuroinflammation

Fig. 7

a Representative immunostaining images of presynaptic marker VGlut2 (red), lysosomal marker CD68 (magenta), and microglial marker Iba1 (green) in the hippocampal CA1 regions, the corresponding 3D construction of VGlut2 (red), CD68 (magenta), and Iba1 (green) channels by Imaris, as well as 3D reconstructed images of the microglial lysosome contents (magenta) and engulfed synaptic contents (red). Scale bar = 10 μm. b Quantification of the percentage of engulfed VGlut2+ synaptic volume in Iba1+ microglial volume (n = 6 images from 3 mice for each group. F (3, 20) = 25.95, P < 0.0001). All data are presented as the mean ± SEM. One-way ANOVA with Tukey’s post hoc test was applied. ***P  <  0.001, versus Con group. ###P  <  0.001, versus RSP group.

Back to article page