Fig. 1: GHS-R1a and D2R formed heterodimers in vitro and in vivo. | Acta Pharmacologica Sinica

Fig. 1: GHS-R1a and D2R formed heterodimers in vitro and in vivo.

From: Quinpirole ameliorates nigral dopaminergic neuron damage in Parkinson’s disease mouse model through activating GHS-R1a/D2R heterodimers

Fig. 1

a Representative images for GHS-R1a and D2R co-expressing in PC-12 cells; b Measurement of PLA-positive dots for GHS-R1a/D2R heterodimers in PC-12 cells; c Representative confocal microscopy images for D2R-EYFP (top, red), GHS-R1a-ECFP (middle, green) and FRET pseudo-color ratio (GHS-R1a-ECFP/D2R-EYFP) (bottom), arrows indicate FRET imaging; d Measurement of PLA-positive dots for GHS-R1a/D2R heterodimers in the SN and IC of mice; e Identification of neurons co-expressing GHS-R1a and D2R in the SN, IC and VTA of mice; f Heterodimers of GHS-R1a and D2R were measured by BRET. Data are depicted as bar graphs with mean ± SEM. ***P < 0.001, n = 6.

Back to article page