Fig. 2: Knockdown of RIPK1 further increases the Hsp70.1B protein levels in the astrocytes of the ischemic cerebral cortex of rats. | Acta Pharmacologica Sinica

Fig. 2: Knockdown of RIPK1 further increases the Hsp70.1B protein levels in the astrocytes of the ischemic cerebral cortex of rats.

From: RIPK1 inhibition contributes to lysosomal membrane stabilization in ischemic astrocytes via a lysosomal Hsp70.1B-dependent mechanism

Fig. 2

a, b Western blotting results show that the protein levels of Hsp70.1B were increased in the cerebral cortex from 1 to 12 h after pMCAO, and RIPK1 knockdown further increased the protein levels of Hsp70.1B at 6 h post-pMCAO. Columns represent quantitative analysis of immunoblots. Means ± SDs, n = 3. **P < 0.01. c, d RIPK1 knockdown increases the Hsp70.1B levels in astrocytes of the ischemic cerebral cortex. c Representative photomicrographs of double immunostaining of Hsp70.1B and GFAP in the ischemic cortex (Hsp70.1B: red; GFAP: green; Hoechst: blue). GFAP immunolocalization was visualized with astrocyte markers. Scale bars indicate 50 μm. Magnified images are cropped sections from the areas indicated with white borders, and scale bars indicate 10 μm. d Quantitative analysis of Hsp70.1B fluorescence intensity in c. Means ± SDs, n = 6. **P < 0.01. Statistical analysis was performed with one-way ANOVA followed by a post hoc Tukey test.

Back to article page