Fig. 3: Knockdown of RIPK1 increases the Hsp70.1B levels in astrocytes with OGD-induced injury. | Acta Pharmacologica Sinica

Fig. 3: Knockdown of RIPK1 increases the Hsp70.1B levels in astrocytes with OGD-induced injury.

From: RIPK1 inhibition contributes to lysosomal membrane stabilization in ischemic astrocytes via a lysosomal Hsp70.1B-dependent mechanism

Fig. 3

a, b Western blotting results show that the protein levels of Hsp70.1B were increased in OGD-treated astrocytes from 1 to 12 h (a), and RIPK1 knockdown further increased the protein levels of Hsp70.1B in astrocytes at 6 h post-OGD (b). Columns represent quantitative analysis of immunoblots. Means ± SDs, n = 3. **P < 0.01. c RIPK1 knockdown increases the Hsp70.1B protein levels in the lysosomes of ischemic astrocytes. Representative photomicrographs of double immunostaining of Lamp1 and Hsp70.1B in astrocytes (Lamp1: red; Hsp70.1B: green; Hoechst: blue). Scale bars indicate 50 μm. Magnified images are cropped sections from the areas indicated with white borders, and scale bars indicate 10 μm. Columns represent quantitative analysis of the Lamp1 and Hsp70.1B fluorescence intensity after immunostaining. Manders’ overlap coefficient demonstrates the colocalization between Hsp70.1B and Lamp1. Means ± SDs, n = 6. *P < 0.05, **P < 0.01. Statistical analysis was performed with one-way ANOVA followed by a post hoc Tukey test.

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