Fig. 4: Hsp70.1B knockdown decreases lysosomal membrane integrity and blocks Nec-1-mediated protection of lysosomal membranes. | Acta Pharmacologica Sinica

Fig. 4: Hsp70.1B knockdown decreases lysosomal membrane integrity and blocks Nec-1-mediated protection of lysosomal membranes.

From: RIPK1 inhibition contributes to lysosomal membrane stabilization in ischemic astrocytes via a lysosomal Hsp70.1B-dependent mechanism

Fig. 4

a, b Nec-1-mediated increase in the Hsp70.1B levels is abolished by Hsp70.1B knockdown in the ischemic cerebral cortex of rats (a) or in OGD-treated astrocytes (b). Columns represent quantitative analysis of immunoblots. Means ± SDs, n = 3. *P < 0.05, **P < 0.01. c Representative photomicrographs of AO staining. Cells were subjected to OGD for 6 h and then incubated with AO (5 μg/mL) for 15 min. Nec-1 (100 μM) was added to the cells 30 min before OGD. Scale bars indicate 50 μm. Magnified images are cropped sections from the areas indicated with white borders, and scale bars indicate 5 μm. Columns represent quantitative analysis of red fluorescence intensity of AO staining. Means ± SDs, n = 6. **P < 0.01. Statistical analysis was performed with one-way ANOVA followed by a post hoc Tukey test.

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