Fig. 9: Knockdown of RIPK1 inhibits the interaction between Hsf1 and Hsp90. | Acta Pharmacologica Sinica

Fig. 9: Knockdown of RIPK1 inhibits the interaction between Hsf1 and Hsp90.

From: RIPK1 inhibition contributes to lysosomal membrane stabilization in ischemic astrocytes via a lysosomal Hsp70.1B-dependent mechanism

Fig. 9

a, c RIPK1 knockdown decreases the Hsp90 level in the cytoplasm and increases the Hsf1 level in the nucleus in OGD-treated astrocytes, as shown by Western blotting analysis. Columns represent quantitative analysis of immunoblots. GAPDH or Lamin B was used as a loading control in the cytoplasm or nucleus, respectively. Means ± SDs, n = 3. *P < 0.05, **P < 0.01. b, d RIPK1 knockdown decreases the interaction between Hsf1 and Hsp90 in OGD-treated cells. Cell lysates were subjected to immunoprecipitation with an Hsf1 antibody, and the level of Hsp90 in the immune complex was determined by Western blotting. Columns represent quantitative analysis of immunoblots. Levels of β-actin protein were used as the loading control. Means ± SDs, n = 3. **P < 0.01. e qPCR results show that RIPK1 knockdown upregulates Hsp70.1B mRNA expression in OGD-treated astrocytes. Means ± SDs, n = 3. *P < 0.05, **P < 0.01. Statistical analysis was performed with one-way ANOVA followed by a post hoc Tukey test.

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