Fig. 9: Autotaxin activity and expression are both reduced in IND-treated mice. | Acta Pharmacologica Sinica

Fig. 9: Autotaxin activity and expression are both reduced in IND-treated mice.

From: A dual role of lysophosphatidic acid type 2 receptor (LPAR2) in nonsteroidal anti-inflammatory drug-induced mouse enteropathy

Fig. 9

The effect of IND on autotaxin (ATX) activity and expression. a Activity of ATX in the plasma of wild-type (WT) and Lpar2−/− mice treated with either VEH or IND (20 mg/kg). b The effect of IND (30–300 μM) on FS-3 hydrolysis caused by human recombinant ATX (3–30 nM). c The effect of IND on LPC 18:1 hydrolysis caused by human recombinant ATX (10 nM). d FS-3 hydrolysis in plasma of VEH-treated mice spiked with IND. e, f Expression of ATX protein (plasma) and Enpp2 mRNA (small intestine) in VEH- and IND-treated mice. Values indicate the mean + SEM, circles on (e) and (f) represent the data of each mouse. For statistical analysis two-way ANOVA (a), one-way ANOVA followed by Fisher’s LSD test (b–d, f), or Student t test (e) were used, n = 3–7/group, **P < 0.01, ***P < 0.001 compared to VEH-treated group, ###P < 0.001 compared to the respective WT group.

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