Fig. 4: HBB overexpression enhanced the responsive sensitivity of AC-induced cardiotoxicity.

a, b HBB expressions in heart tissues were evaluated by Western blotting and immunohistochemistry (IHC) (n = 5). c Cell viability assay of cardiomyocytes after exposed AC (n = 3). d The ROS level in primary-isolated cardiomyocytes was quantify(n = 3). e HBB protein expression and purification. f HBB pure proteins were given to mice. (HBB and IgG, 125 mg/kg, i.v, n = 6) (*P < 0.05, **P < 0.01, ***P < 0.001; #P < 0.05, ##P < 0.01).