Fig. 7: HBB promotes AC-induced cardiotoxicity via interfering with ABHD5/AMPK/HDAC4 axis. | Acta Pharmacologica Sinica

Fig. 7: HBB promotes AC-induced cardiotoxicity via interfering with ABHD5/AMPK/HDAC4 axis.

From: HBB contributes to individualized aconitine-induced cardiotoxicity in mice via interfering with ABHD5/AMPK/HDAC4 axis

Fig. 7

a The IP-mass assay was performed to determine HBB-binding proteins in HBB-overexpressed AC16 cells. b CO-IP assay was used to verify the binding of HBB with ADH1, ABHD5, and AMPK. Docking data (c–e) and the protein binding affinities (f) of HBB with ADH1, ABHD5, and AMPK before and after AC interfering. The protein levels of full length HDAC (HDAC-FL) (g), N-terminal polypeptide of HDAC4 (HDAC4-NT) (g), AMPK (h), p-AMPK (h), mTOR (h), and p-mTOR (h) were assessed (n = 3). i The protein levels of AMPK, ADH1, and ABHD5 were detected by IHC.

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