Fig. 3

CANA alleviates Su/Hx/Nx mouse PH. a Flowchart for Su/Hx/Nx mouse PH model establishment. b Quantitative analysis of the RVSP in Nx, Su/Hx/Nx and Su/Hx/Nx+CANA groups. c Quantitative analysis of the mCAP in these groups (n = 6 mice per group). Quantitative analysis of RV/(S+LV) (d) and RV/BW (e) in these groups (n = 6 mice per group). Echocardiography measurements illustrating PAT/PET (f), PAT (g), PAVTI (h) and RVFWT (i) of these three groups (n = 6 mice per group). j Representative images of H&E staining, immunohistochemistry and Masson staining in Nx, Su/Hx/Nx and Su/Hx/Nx+CANA groups. Quantitative analysis of WA/TA (%) (k), WT/TT (%) (I), media thickness index (m), and the rate of fibrosis area (n) of each group as shown in (j). n = 6 mice per group, four images per section. ^nsP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Scale bars = 20 μm.