Fig. 4

CANA promotes PPARγ expression, and inhibits excessive oxidative stress and proliferation in vivo. a Volcano plots showing the differences between Su/Hx and Su/Hx+CANA groups. b KEGG enrichment analysis of different expressed genes. c GO enrichment analysis of different expressed genes. d Upper panel: Representative chemiluminescence detection images detected PPARγ expression levels in lung tissue homogenates of Nx, Su/Hx and Su/Hx+CANA groups. Bottom panel: Quantitative analysis of PPARγ relative to β-ACTIN (n = 6 mice per group). e qPCR analysis of NRF2, CAT, HO-1, PPARγ, PGC1a, and SOD2 expression in mouse lung tissue of these groups (n = 6 mice per group). f–i Measurement of lung tissue homogenates of SOD, CAT. MPO, and MDA activity in these groups (n = 6 mice per group). j DHE staining assay was performed in fresh lung frozen sections of these groups. k Quantification of DHE fluorescence intensity averaged on each group (n = 6 mice per group, three images per section). l Detection of PCNA in PASMCs of Nx, Su/Hx and Su/Hx+CANA group by immunohistochemistry staining assay. m Quantification of PCNA⁺ cells /total PASMCs on each group (n = 6 mice per group, four images per section). ^nsP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Scale bars = 20 μm.