Fig. 6

CANA inhibits rPASMCs excessive oxidative stress and proliferation by promoting PPARγ expression. a Upper panel: Chemiluminescence detection images detected PPARγ and Hif-1α expression levels in cultured rPASMCs under CANA (0, 2, 5, 10, 20 μM) treatment after hypoxia exposure for 24 h. Bottom panel: Quantitative analysis of PPARγ (n = 6 independent experiments). b Upper panel: Chemiluminescence detected PPARγ expression levels in rPASMCs transfected with three different siRNA, compared with NC group. Bottom panel: Quantitative analysis of PPARγ (n = 6 independent experiments). c Upper panel: Chemiluminescence detected PCNA, PPARγ and Hif-1α expression levels in rPASMCs treated with CANA and with or without transfected PPARγ siRNA. Bottom panel: Quantitative analysis of PPARγ (n = 6 independent experiments). d EdU staining in rPASMCs of each group. e Quantitative analysis of EdU⁺ cells in each group (n = 6 sections from six independent experiments, three images per section). f Intracellular ROS reacted with DCFH-DA and highly fluorescent DCF was detected by flow cytometry. g Quantification of DCF fluorescence on cells of each group (n = 6 per group from six independent experiments). ^nsP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Scale bars = 20 μm.