Fig. 3: Altered microglial response and phenotype in FGF21−/− mice after stroke. | Acta Pharmacologica Sinica

Fig. 3: Altered microglial response and phenotype in FGF21−/− mice after stroke.

From: FGF21, a modulator of astrocyte reactivity, protects against ischemic brain injury through anti-inflammatory and neurotrophic pathways

Fig. 3

a Microphotographs and bar graph b of Iba1-positive (red) microglia in the perifocal cortex of WT and FGF21−/− mice show the changes in microglia density at 3 and 14 d after tMCAO. n = 8/group. c FACS analysis and quantitative data of the expression of CD68, CD86, and CD206 in microglia (CD45intCD11b+) by FMO control. n = 6/group. *P < 0.05, **P < 0.01 by 2way-ANOVA with Sidak’s multiple comparison. d FACS images show the purity of microglia (> 99%) after cell sorting. e–k qRT‒PCR shows the mRNA expression of the microglial signature gene (Cd68, Cd86, and Cd206) and inflammatory cytokine (Il-1β, Tnf-α, Il-6, and Tgf-β) in the sorted microglia from the contralesional and ipsilesional brain of WT and FGF21−/− mice. n = 6/group. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, determined by 2way-ANOVA with Tukey’s multiple comparison. Results are presented as mean ± SD.

Back to article page