Fig. 3: DHC suppresses the platelet-derived growth factor-BB (PDGF-BB)-induced VSMC secretory phenotype transition in vitro. | Acta Pharmacologica Sinica

Fig. 3: DHC suppresses the platelet-derived growth factor-BB (PDGF-BB)-induced VSMC secretory phenotype transition in vitro.

From: Dehydrocorydaline maintains the vascular smooth muscle cell contractile phenotype by upregulating Spta1

Fig. 3

a–c Rat VSMCs were exposed to 100 μM DHC and/or 20 ng/mL PDGF-BB for 24 h. a mRNA levels of contractile markers (Cnn1, Myh11, Sm22α, and Acta2) were assessed by RT-qPCR (n = 3). b Protein levels of contractile markers (Cnn1 and SM-MHC) were detected by Western blotting (n = 10). c Protein levels of α-SMA (green) were detected by immunofluorescence, and DAPI (blue) indicates the nucleus (n = 4, scale = 50 μm). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, analyzed using one-way ANOVA.

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