Fig. 1: Screening and identification of OGT and CDC27 as key hub genes in MM. | Acta Pharmacologica Sinica

Fig. 1: Screening and identification of OGT and CDC27 as key hub genes in MM.

From: Inhibition of CDC27 O-GlcNAcylation coordinates the antitumor efficacy in multiple myeloma through the autophagy-lysosome pathway

Fig. 1

a Flowchart shows the machine learning strategy. b Volcano plot shows the differentially expressed genes between healthy controls (HC) and multiple myeloma (MM) patients. c Venn diagram shows the common hub genes from three machine learning models. d ROC curves show the hub genes in training set (upper) and testing set (lower). e Violin plots show the expression levels of OGT and CDC27 genes in HC and MM patients. f Plot shows survival curves in MM patients with high or low OGT gene expression levels. g Representative images and plots show the localization of OGT (red) and O-GlcNAcylation (O-GlcNAc, green) in HC CD138+ plasma cells and MM.1S cells. Scale bar = 10 μm. h, i Bar charts show the MFI values of OGT and O-GlcNAc in the total, cytoplasmic, and nuclear regions of HC and MM cells. Data were obtained from two independent experiments in g–i. Data are presented as the means ± SD. Two-tailed Mann‒Whitney U test in e. Unpaired two-tailed Student’s t test in h, i. ns: not significant. *P < 0.05, **P < 0.01, ***P < 0.001.

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