Fig. 4: O-GlcNAcylation maintains the CDC27 protein stability through the autophagy-lysosome pathway (ALP) in MM cells. | Acta Pharmacologica Sinica

Fig. 4: O-GlcNAcylation maintains the CDC27 protein stability through the autophagy-lysosome pathway (ALP) in MM cells.

From: Inhibition of CDC27 O-GlcNAcylation coordinates the antitumor efficacy in multiple myeloma through the autophagy-lysosome pathway

Fig. 4

a qPCR analysis of CDC27 mRNA levels in MM.1S cells treated with OSMI-1 (upper) or with Thiamet G (lower). b Representative images and plots show the half-life of the CDC27 protein in OSMI-1-treated (upper) or Thiamet G-treated (lower) MM.1S cells incubated with cycloheximide (CHX) for the indicated durations. c Images and statistical analysis show the protein levels of CDC27, LAMP1, p62, β5, LC3 in MM.1S cells treated with or without bortezomib (BTZ), 3-methylamphetamine (3-MA), or chloroquine (CQ) in the absence or presence of OSMI-1 for 24 h. d Representative images show the binding between CDC27 and LAMP1, p62, β5, and LC3II/I in OSMI-1-treated MM.1S cells via the Co-IP assay. e Representative images and plots show the colocalization of CDC27 (red) and LC3 (green) in OSMI-1-treated MM.1S cells by confocal microscopy. Scale bar = 10 μm. The data were obtained from three independent experiments. The data are presented as the means ± SD. Unpaired two-tailed Student’s t-test was used in a, b, c, and e. ns: not significant. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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