Fig. 1: Identification of circRNAs derived from SARS-CoV-2 nucleocapsid gene. | Acta Pharmacologica Sinica

Fig. 1: Identification of circRNAs derived from SARS-CoV-2 nucleocapsid gene.

From: CircSARS-CV2-N1368 from SARS-CoV-2 impairs endothelial cell function through the upregulation of ATF7 to activate TLR4/NF-κB/ROS signaling

Fig. 1

a Genome organization of SARS-CoV-2, and circSARS-CV2-Ns generated from SARS-CoV-2 N gene. b PCR products of circSARS-CV2-Ns by agarose gel electrophoresis. c Detection of SARS-CV2-Ns in pharyngeal swab samples from patients with COVID-19 by RT-qPCR assay (1-way ANOVA, n = 5 per group). d RNA FISH assay of circSARS-CV2-N654, -N804, and -N1368 in human cardiomyocyte AC16. CircRNAs were shown in red and nuclei were stained with DAPI. The scale bar is 25 μm. e Levels of circSARS-CV2-N654, -N804, -N1368 and GAPDH mRNA in RNase R-treated total RNA (20 U/mg RNA) from AC16 cells were detected by RT-qPCR assay (unpaired t test, n = 3 per group). *P < 0.05; **P < 0.01; ***P < 0.001.

Back to article page