Fig. 3: Effects of circSARS-CV2-Ns overexpression on endothelium-dependent vasorelaxation and phenotypes of HCMECs. | Acta Pharmacologica Sinica

Fig. 3: Effects of circSARS-CV2-Ns overexpression on endothelium-dependent vasorelaxation and phenotypes of HCMECs.

From: CircSARS-CV2-N1368 from SARS-CoV-2 impairs endothelial cell function through the upregulation of ATF7 to activate TLR4/NF-κB/ROS signaling

Fig. 3

a Detection of acetylcholine-induced endothelium-dependent vascular relaxation in rat coronary arteries with overexpression of circSARS-CV2-Ns (1-way ANOVA, n = 4 per group). b VEGFA expression and e-NOS activation (eNOS(S1177) phosphorylation) in rat coronary arteries by Western blot assay. c VEGFA expression, e-NOS and HH3 activation (phosphorylation of HH3) in circSARS-CV2-Ns-overexpressiong HCMECs by Western blot assay (1-way ANOVA, n = 3 per group). d Proliferation activity analysis of HCMECs by EdU assay (1-way ANOVA, n = 3 per group). Migration activity analysis of HCMECs by trans-well migration assay (e) and wound healing assay (f) (1-way ANOVA, n = 3 per group), respectively. g Microscopic images showing matrigel tube formation of HCMECs with overexpression of circSARS-CV2-Ns (1-way ANOVA, n = 3 per group). h Detection of ROS level by using DCFH-DA probe (1-way ANOVA, n = 3 per group). i Effect of exogenous overexpression of circSARS-CV2-Ns on angiogenesis in vivo by matrigel plug assay (1-way ANOVA, n = 5 per group). j, k Effects of exogenous overexpression of circSARS-CV2-Ns on size and beat of COs (2-way ANOVA, n = 5 per group). l Detection of eNOS and VEGFA mRNA expression by RT-qPCR assay (2-way ANOVA, n = 3 per group). The scale bar is 50 μm in (d), (e), (h), (i), the scale bar is 100 μm in (g), the scale bar is 200 μm in (f), (j). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

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