Fig. 5: CircSARS-CV2-N1368 inhibits proliferation, migration and tube-formation of HCMECs via sponging miR-103a-3p. | Acta Pharmacologica Sinica

Fig. 5: CircSARS-CV2-N1368 inhibits proliferation, migration and tube-formation of HCMECs via sponging miR-103a-3p.

From: CircSARS-CV2-N1368 from SARS-CoV-2 impairs endothelial cell function through the upregulation of ATF7 to activate TLR4/NF-κB/ROS signaling

Fig. 5

a Effect of miR-103a-3p on expression of VEGFA, e-NOS and HH3 activation in circSARS-CV2-N1368-overexpressiong HCMECs by Western blot assay. b MiR-103a-3p decreased ROS level in circSARS-CV2-N1368 overexpressing HCMECs. c Proliferation activity analysis of HCMECs by EdU assay. d, e Migration activity analysis of HCMECs by trans-well migration assay and wound healing assay, respectively. f Matrigel tube formation assay of circSARS-CV2-Ns-overexpressiong HCMECs with transfection of miR-103a-3p. Comparisons were made with 1-way ANOVA, n = 3 per group in (b)–(f). The scale bar is 50 μm in (b), (c), (e), the scale bar is 100 μm in (f), the scale bar is 200 μm in (d). *P < 0.05, **P < 0.01, ***P < 0.001.

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