Fig. 6: CircSARS-CV2-N1368 regulates the phenotypes of HCMECs by targeting miR-103a-3p/Atf7 axis. | Acta Pharmacologica Sinica

Fig. 6: CircSARS-CV2-N1368 regulates the phenotypes of HCMECs by targeting miR-103a-3p/Atf7 axis.

From: CircSARS-CV2-N1368 from SARS-CoV-2 impairs endothelial cell function through the upregulation of ATF7 to activate TLR4/NF-κB/ROS signaling

Fig. 6

a Venn diagram showing the overlapped genes. b and c The expression of the concerned genes in HCMECs with transfection of miR-103a-3p by RT-qPCR assay. d Validation of the interaction between miR-103a-3p and Atf7 mRNA by RIP assay. e Determination of ATF7 and VEGFA expression, activations of e-NOS, HH3 and NF-κB p65 in HCMECs by Western blot assay. f MiR-103a-3p and si-Atf7 decreased ROS generation in circSARS-CV2-N1368 overexpressing HCMECs. g Proliferation activity analysis of HCMECs by EdU assay. h, i Migration activity of HCMECs by trans-well migration assay and wound healing assay, respectively. j Matrigel tube formation assay of circSARS-CV2-Ns-overexpressiong HCMECs with transfection of miR-103a-3p or si-Atf7. Comparisons were made with unpaired t test in (b)–(d), and comparisons were made with 1-way ANOVA in (e)–(i), n = 3 per group. The scale bar is 50 μm in (f)–(h), the scale bar is 100 μm in i, the scale bar is 200 μm in (j). **P < 0.01, ***P < 0.001.

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