Fig. 6: HuR regulates E2F7 by stabilizing its mRNA.
From: Targeting the HuR/E2F7 axis synergizes with bortezomib against multiple myeloma
a HuR-knockdown H929 and OPM2 cells were treated with actinomycin D for 0, 2, 4, and 6 h. E2F7 mRNA levels were measured at corresponding time points via qRT‒PCR. b, c RIP‒qPCR was performed to detect the interaction between HuR and E2F7 mRNA in NCI-H929 and OPM2 cells. d The interaction between HuR protein and E2F7 mRNA in NCI-H929 and OPM2 cells was detected by RIP-PCR and agarose gel electrophoresis. e The 3′-UTR sequence of E2F7 mRNA containing the HuR binding site is shown, and this site was predicted by an online website (https://rbpmap.technion.ac.il/index.html). CDS: coding sequence. f HEK293T cells were cotransfected with luciferase reporter plasmids (psiCHECK2, E2F7 3′-UTR wt, or E2F7 3′-UTR mut) and plvx-HuR or plvx-NC plasmids. Luciferase activity was measured 48 h after transfection. The data are shown as the means ± SDs, and statistical analysis was performed via Student’s t-test (b, c) or one-way ANOVA (a, f). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
