Fig. 2: Cardiomyocyte-specific circDhx32 knockdown relieves myocardial impairment in I/R model mice.

a Schematic diagram showing that mice were injected with the AAV9 vector carrying an NC-shRNA or circDhx32-shRNA fragment via the tail vein, after which the I/R model was established and harvested at the corresponding time points. b qRT‒PCR was performed to test circDhx32 expression. n = 5‒6. ^**P < 0.01 vs. Sham+shNC-V; ^##P < 0.01 vs. I/R+shNC-V. One-way ANOVA followed by Dunnett’s multiple comparisons test was performed to evaluate significant differences. c‒e Representative images of echocardiographs and statistics of EF and FS. n = 6‒7. ^**P < 0.01 vs. Sham+shNC-V; ^##P < 0.01 vs. I/R+shNC-V. One-way ANOVA followed by Dunnett’s multiple comparisons test was performed to evaluate significant differences. f Evans blue/TTC staining was used to evaluate infarct size in I/R-treated mouse hearts. n = 8‒9. ^**P < 0.01 vs. I/R+shNC-V. One-way ANOVA followed by Dunnett’s multiple comparisons test was performed to evaluate significant differences. g Detection of plasma CK-MB levels in the mice. n = 6‒7. ^**P < 0.01 vs. Sham+shNC-V; ^##P < 0.01 vs. I/R+shNC-V. One-way ANOVA followed by Dunnett’s multiple comparisons test was performed to evaluate significant differences. h Concentration of cTnT in mouse serum. n = 6‒8. ^**P < 0.01 vs. Sham+shNC-V; ^##P < 0.01 vs. I/R+shNC-V. One-way ANOVA followed by Dunnett’s multiple comparisons test was performed to evaluate significant differences. i Representative images of H&E staining of the myocardium. Scale bar = 20 μm. n = 6‒8.