Fig. 3: CircDhx32 downregulation alleviates cardiomyocyte injury upon H/R treatment.

a Schematic diagram of circDhx32 siRNA. b Relative circDhx32 expression after transfection of circDhx32 siRNA (si-circDhx32) or NC siRNA (si-NC). n = 6. ^**P < 0.01 vs. NC. P values were determined via an unpaired t test. c mRNA expression level of Dhx32 in cardiomyocytes. n = 6. P values were determined via an unpaired t test. d Cell viability was examined by CCK-8 assays of cardiomyocytes after H/R. n = 6. ^**P < 0.01 vs. si-NC; ^##P < 0.01 vs. H/R+si-NC. One-way ANOVA followed by Dunnett’s multiple comparisons test was performed to evaluate significant differences. e Detection of LDH release in cardiomyocytes after H/R. n = 6. ^**P < 0.01 vs. si-NC; ^##P < 0.01 vs. H/R+si-NC. One-way ANOVA followed by Dunnett’s multiple comparisons test was performed to evaluate significant differences. f‒h Representative images of live and dead cell staining and data analysis of the death ratio and live ratio (%). Scale bar = 50 μm. n = 4. ^**P < 0.01 vs. si-NC; ^##P < 0.01 vs. H/R+si-NC. One-way ANOVA followed by Dunnett’s multiple comparisons test was performed to evaluate significant differences.