Fig. 1: Impairment of the cGAS-STING signaling pathway in tumor cells. | Acta Pharmacologica Sinica

Fig. 1: Impairment of the cGAS-STING signaling pathway in tumor cells.

From: DNMT inhibition epigenetically restores the cGAS-STING pathway and activates RIG-I/MDA5-MAVS to enhance antitumor immunity

Fig. 1

a Schematic diagrams of the dsDNA and dsRNA sensing pathways. b Immunoblot analysis of innate immune signaling pathway-related protein expression levels in different breast cancer cell lines. c Bioinformatic analysis of cGAS and STING expression levels in breast cancer cell lines from the HPA database, with BT549 and MDA-MB-436 representing the upper range of expression. d Representative IHC staining images showing low and high expression of cGAS and STING in breast cancer tissue arrays. e Immunoblot analysis of the indicated proteins in various breast cancer cell lines transfected with 0.5 μg/mL Poly (dA:dT) or Poly (I:C) for 4 h. f RT-qPCR analysis of IFNB, and CXCL10 levels in different breast cancer cell lines transfected with 0.5 μg/mL Poly (dA:dT) or Poly (I:C) for 4 h. **P < 0.01, ***P < 0.001, ****P < 0.0001, ns no significant difference.

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