Fig. 3: DNMT inhibitor can relieve the repression of cGAS and STING. | Acta Pharmacologica Sinica

Fig. 3: DNMT inhibitor can relieve the repression of cGAS and STING.

From: DNMT inhibition epigenetically restores the cGAS-STING pathway and activates RIG-I/MDA5-MAVS to enhance antitumor immunity

Fig. 3

a Immunoblot analysis of the indicated proteins in A549 cells treated with the indicated epigenetic drugs. b Immunoblot analysis of the indicated proteins in MDA-MB-453 cells treated with 0.5 μM DAC at different times. Immunoblot analysis of the indicated proteins in A549 (c) and MDA-MB-453 (d) cells treated with DAC for 72 h. e RT-qPCR analysis of cGAS and STING levels in MDA-MB-453 cells treated with DAC for 72 h. IF images of MDA-MB-231 cells stained with DAPI (blue), cGAS (green) (f) and STING (green) (g) after treatment with 2 μM DAC for 72 h. *P<0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns no significant difference.

Back to article page