Fig. 4: ALP staining in experimental groups and control groups.
From: Mega-oss and Mega-TCP versus Bio-Oss granules fixed by alginate gel for bone regeneration
Alkaline phosphatase (ALP) staining of MC3T3-E1 cells in the experimental groups and control groups. MC3T3-E1 cells were cultured with or without differentiation for 7 d and stained with BCIP/NBT. a Mega-oss with differentiation; b Mega-oss without differentiation; c Mega-TCP with differentiation; d Mega-TCP without differentiation; e Bio-Oss with differentiation; f Bio-Oss without differentiation; g Sodium alginate with differentiation; h Sodium alginate without differentiation; i only cells with differentiation; j only cells without differentiation. BCIP/NBT, 5-bromo-4-chloro-3′-indolyphosphate p-toluidine salt/nitro-blue tetrazolium chloride.
