Fig. 2: Cell proliferation of ihDPs.
From: Betaine promotes osteogenic differentiation in immortalized human dental pulp-derived cells
Cell proliferation was evaluated using an MTT assay on days 1, 3, and 7 (A). Cell cycle analysis was performed using flow cytometry (B, C). Colony-forming unit assay. Scale bars: 300 µm (D). Senescence-associated β-galactosidase activity. Scale bars: 300 µm (E). The inset images showed the β-galactosidase-positive cells. Bars indicate a significant difference between groups (*P < 0.05, **P < 0.01).
