Fig. 2: Preparation of platelet-rich fibrin clot and its placement in the root canal.

A The three distinct blood layers of centrifuged blood samples at 3000 rpm for 10 min after being collected into sterile, plain-type, single-use centrifuge tubes. B Isolated yellow layer of PRF with an HTS 171c66.25 curved stainless steel tweezer. C The isolated PRF clot cut into smaller fragment. D, E PRF clot fragments were immediately introduced into the root canal using a small hand plugger. F a 3 mm layer thickness of Biodentine (Biodentine®, packed over the PRF.