Fig. 1

Gene and miRNA expression profiles of PV and ET CD34+ cells according to JAK2 and CALR mutational status. a, b Principal component analysis (PCA) on gene and miRNA expression microarray data. The unsupervised PCA analysis on global gene expression a and miRNA expression data b were computed using Partek GS, version 6.6. BM control samples are shown as red spheres; PV samples are shown in green; JAK2V617F-positive ET samples are shown in blue; CALR-mutated ET samples are shown in orange; c, d The heat maps were computed on the gene list of DEG c and DEM d (provided in Supplementary Tables 1 and 2, respectively) using the clustering algorithm included in the Partek GS package by means of euclidean distance and average linkage. Gene coloring is based on normalized signals, as shown on the left; green indicates reduced expression, red increased expression in the pairwise comparison CALR-mutated vs. JAK2V617F-positive ET. CALR-mutated ET groups clustered separately in the dendrogram shown at the top of the heat map. e Ingenuity pathway analysis (IPA) of modulated genes in CALR-mutated ET vs. JAK2V617F-positive ET. The graph shows the most highly represented canonical pathways in the list of DEG and their overlapping connections