Table 1 Clonal hematopoiesis mutations discovered in the study

Pt. ID	Age at sampling	Gene	Mutation	AA change	VAF	SIFT/Polyphen	Smoking history	Cancer	Medication before sampling	Max MTX dose (mg/week)
RA1	70	GNAS	20:g.57428923G>A	S138N	0.021	0.19/0.27	Ex-smoker	No	MTX, OXI, iaCS	25
RA2	72	TET2	4:g.106155319_106155320insT	V74fs	0.048	NA	Never	No	MTX, OXI, iaCS	25
RA2	36	CBL	11:g.119149250C>T	R420X	0.022	NA	Smoker	No	MTX, OXI, iaCS	25
RA3	68	GNAS	20:g.57429086G>A	A256T	0.027	0.16/0.001	Ex-smoker	No	MTX, OXI, SA, CS, iaCS, golimumab, etanercept	25
RA4	61	TET2	4:g.106157845C>T	Q916X	0.10	NA	Ex-smoker	No	MTX, OXI	10
RA5	67	DNMT3A	2:g.25459850_25459856delATCATTC	V809fs	0.031	NA	Smoker	No	MTX, OXI, SA, CS	25
RA6	55	TP53	17:g.7574003G>A	R342X	0.0432	NA	Smoker	Yes^a	MTX, OXI, SA, CS	25
RA7	65	DNMT3A	2:g.25467117G>T	C586X	0.061	NA	Ex-smoker	No	MTX, OXI, CS	25
RA8	70	TET2	4:g.106197060C>G	S1798X	0.057	NA	Smoker	No	MTX, iaCS, abatacept	20
RA9	59	DNMT3A	2:g.25470590A>G	L295P	0.036	0/1	Never	No	MTX, OXI	20
RA9	70	ASXL1	20:g.31022367A>T	K618X	0.077	NA	Ex-smoker	No	MTX, OXI	20
RA10	72	DNMT3A	2:g.25462038C>T	R790K	0.044	0/0.88	Never	No	none	NA
AA1	53	LAMB4	7:g.107688489C>T	R1397Q	0.021	0.63/0	Ex-smoker	No	CS	NA
AA1	53	LAMB4	7:g.107688489C>T	R1397Q	0.039	0.63/0	Ex-smoker	No	CS + CyA	NA
AA2	54	PIGA	X:g.15342786_15342786delC	Splice; impact high	0.13	NA	Never	No	none	NA
AA4	64	ASXL1	20:g.31022288C>A	Y591X	0.14	NA	Smoker	No	none	NA
AA4	64	TET2	4:g.106157409_106157413delAAGAG	Q770fs	0.16	NA	Smoker	No	none	NA
MDS4	57	STAG2	X:g.123202468_123202468delC	H774fs	0.051	NA	Ex-smoker	No	none	NA

Table 1 shows information on patients with clonal hematopoiesis. Additional patient information is presented in the Supplementary material. The mutations are shown in HGVS format in the Hg19 reference genome coordinate system. The amino acid change is shown for the canonical transcript (the CCDS transcript with the longest translation) or for the transcript in which the mutation has the highest impact. Transcript IDs and more detailed information are shown in Supplementary table 2. SIFT and Polyphen scores are prediction scores for missense mutations on the effect for the protein. Both are scaled from 0 to 1. For SIFT, 0 corresponds to deleterious and 1 tolerated. For Polyphen, 1 corresponds to damaging and 0 benign. Disease-modifying medication history before the samples were obtained is included in the table. AA, amino acid; VAF, variant allele frequency; NA, not applicable; Max, maximum; MTX, methotrexate; OXI, hydroxychloroquine; SA, sulfasalazine; CS, corticosteroids; iaCS, intra-articular corticosteroids; CyA, cyclosporine A
^aCervical cancer treated with surgery. The patient did not receive chemotherapy, or radiotherapy

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