Fig. 1: Characterization of extracellular vesicles. | Blood Cancer Journal

Fig. 1: Characterization of extracellular vesicles.

From: Exosomes play a role in multiple myeloma bone disease and tumor development by targeting osteoclasts and osteoblasts

Fig. 1

a Nanoparticle tracking analysis by Zetaview of isolated EVs show a mean diameter of 114.4 nm (SD 36.9 nm) with a maximum diameter of 153 nm; analysis of zeta potential show a negative potential of these vesicles. The Zetaview was calibrated using standard beads of 100 nm. EVs were dissolved in PBS and PBS was used as a negative control. 11 frames per sample were analyzed. b Transmission electron microscopy analysis of sEVs isolated by ExoQuick protocol show the presence of vesicles with the appropriate size and morphology. c Western Blot analysis of extracellular vesicles show the absence of calreticulin (intracellular protein, absent in EVs/exosomes) and presence of tetraspanins CD81 and CD63 as well as endosomal markers TSG101 and Syntenin, enriched in exosomes. One experiment representative of 3 is shown

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