Fig. 2: 5TGM1 sEVs induce osteolysis in vivo in C57Bl6/KalWRij mice. | Blood Cancer Journal

Fig. 2: 5TGM1 sEVs induce osteolysis in vivo in C57Bl6/KalWRij mice.

From: Exosomes play a role in multiple myeloma bone disease and tumor development by targeting osteoclasts and osteoblasts

Fig. 2

a 5-week old C57Bl6/KalWRij mice (n = 6) were injected intravenously three times per week with 200 µg of exosomes, for three weeks. At day 20, all mice were sacrificed and femurs and spleen were isolated. b Spleens of mice injected with 5TGM1 sEVs nearly doubled in size compared to controls injected with PBS. c Representative 3D-reconstruction of trabecular bone of femurs of control mice vs. 5TGM1 sEVs injected mice. d Morphometric µCT analysis shows a significant lower trabecular number (Tb. N) and higher Trabecular separation (Tb Sp.), leading to a significantly lower trabecular bone volume and a lower connectivity density in mice injected with 5TGM1 sEVs. Experiment was repeated twice, once as a pilot study with n = 3. Final experiment is shown (n = 6). ** = p < 0.01

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