Fig. 3: 5TGM1 sEVs aid in differentiation of RAW264.7 cells to osteoclasts, and increase their resorptive activity. | Blood Cancer Journal

Fig. 3: 5TGM1 sEVs aid in differentiation of RAW264.7 cells to osteoclasts, and increase their resorptive activity.

From: Exosomes play a role in multiple myeloma bone disease and tumor development by targeting osteoclasts and osteoblasts

Fig. 3

a Representative images of TRAP-stained RAW264.7 osteoclast cultures incubated with RANKL (30 ng/ml) on day 1 in all conditions. At day 4, medium was refreshed and either 5TGM1 CCM, or 5TGM1 sEVs (100 µg/ml) was added. ×4 and ×10 magnifications are shown. b Representative images of resorption pits (Von Kossa staining) generated by RAW264.7 osteoclasts incubated with either 5TGM1 CCM, 5TGM1 sEVs (100 µg/ml) or RANKL (positive control) (×40 magnification). c Quantification of the matrix resorption area in RAW264.7-derived osteoclast cultures. d Quantification of the number of resorption pits per field of view (N. pits/FOV). e RAW264.7 osteoclast cultures were cultured with 5TGM1 concentrated conditioned medium (CCM) or medium deprived of sEVs. Relative viability after 24 h was determined by a Cell Titer Glo® luminescence assay. Control = serum-free medium. Mean value + SD are shown for 3 independent experiments, *: p < 0.05

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