Fig. 1: Stimulation of the HL cell lines with PD-L1 antibody increases cell survival and proliferation and reduces apoptosis. | Blood Cancer Journal

Fig. 1: Stimulation of the HL cell lines with PD-L1 antibody increases cell survival and proliferation and reduces apoptosis.

From: Reverse signaling via PD-L1 supports malignant cell growth and survival in classical Hodgkin lymphoma

Fig. 1

HL cell lines (HL-428, HL-1236, HL-HDLM2, and HL-KMH2) were starved overnight and plated over either isotype control (IgG1) antibody- or PD-L1 antibody-coated (10 μg/ml) plates for 48 h. a Flow-cytometry analysis showing Annexin/PI staining on four different HL cell lines. b Bar graphs showing the proliferation rate of HL cell lines, in response to incubation with isotype control (blue) or PD-L1 antibody (red). c Apoptotic protein array illustrating the differences between apoptotic proteins and phospho-proteins in the HL-428 cells that are incubated with isotype control or PD-L1 antibody (left panel). Bar graphs show the quantitative analysis of the protein dots using ImageJ (right). d Flow-cytometry analysis (left) displays the intracellular staining with p-ERK and p-P38 antibodies in two HL cell lines incubated with isotype control or PD-L1 antibodies. Bar graphs (right) represent the percentage of the cells that are positive for p-P38 and p-ERK in four different cell lines

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