Fig. 3: The CBFβ-MYH11 oncogene blocks cell differentiation towards megakaryocyte/erythrocyte lineages. | Blood Cancer Journal

Fig. 3: The CBFβ-MYH11 oncogene blocks cell differentiation towards megakaryocyte/erythrocyte lineages.

From: CBFβ-MYH11 interferes with megakaryocyte differentiation via modulating a gene program that includes GATA2 and KLF1

Fig. 3

a Western blots of the CBFβ-MYH11 oncoprotein in the inv(16) AML ME-1 cell line and in the iPSC system before and after dox addition. Arrows point to the endogenous CBFβ and inducible expressed CBFβ-MYH11 protein. Asterisks represent aspecific binding of the antibody. Molecular weight markers in kilodaltons are listed on the left. b Expression levels of the CBFβ-MYH11 oncogene in ME-1 cells and in iPSC without (CTR) or after dox induction of CBFβ-MYH11. c The methodology for iPSC differentiation towards neutrophil, macrophage, and megakaryocyte lineages. d The percentage of cells showing specific myeloid markers after differentiation following the protocols in c. The mean and standard deviation of cell fractions from four independent differentiation experiments are shown. A p-value of <0.05 was considered statistically significant by Wilcoxon signed-rank test: *p < 0.05

Back to article page