Fig. 5: Identification of interactors affected by the CBFβ-MYH11 fusion. | Blood Cancer Journal

Fig. 5: Identification of interactors affected by the CBFβ-MYH11 fusion.

From: CBFβ-MYH11 interferes with megakaryocyte differentiation via modulating a gene program that includes GATA2 and KLF1

Fig. 5

a Western blot analysis of a DNA pull-down experiment in ME-1 cells using CBFβ and RUNX1 antibodies. b Scatterplot showing the CBFβ-MYH11 interactome. Proteins interacting with a RUNX1 motif containing oligo before or after CBFβ-MYH11 knockdown are plotted by their SILAC-ratios in the forward (x-axis) and reverse (y-axis) SILAC experiment. Specific interactors with the RUNX1 motif containing oligo that bind upon CBFβ-MYH11 protein depletion lie close to the diagonal in the upper left quadrant. Interactors for which binding is CBFβ-MYH11 dependent are in the lower right quadrant

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