Fig. 5: Combining OTSSP167 with venetoclax leads to a synergistic anti-lymphoma effect. | Blood Cancer Journal

Fig. 5: Combining OTSSP167 with venetoclax leads to a synergistic anti-lymphoma effect.

From: Maternal embryonic leucine zipper kinase is a novel target for diffuse large B cell lymphoma and mantle cell lymphoma

Fig. 5

a Apoptosis of OTSSP167 (ots) and venetoclax-treated SU-DHL-6, U2932, and Jeko-1 cells was determined after 48 h using an Annexin V/7’AAD staining followed by flow cytometry analysis. The sum of the percentage Annexin V and Annexin V/7’AAD positive cells are shown. Results shown are mean ± SD of four independent experiments. Each combination was compared with both single agents. ***p < 0.001 and combination indexes (CI) are shown. b Protein levels of MELK, (p)Bcl-2, (p)Bcl-xL, Mcl1, and caspase-3 were determined in DLBCL and MCL cell lines after 24 h of OTSSP167 treatment in combination with venetoclax. SU-DHL-6, U2932, and Jeko-1 cells were treated with, respectively, 30 nM OTSSP167 and 100 nM venetoclax, 15 nM OTSSP167 and 4.5 nM venetoclax, and 15 nM OTSSP167 and 500 nM venetoclax. β-actin was used as loading control. One experiment representative of 3 is shown.

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