Fig. 2: Aging-associated genes were upregulated in MDS-derived erythroid cells during ex vivo differentiation. | Blood Cancer Journal

Fig. 2: Aging-associated genes were upregulated in MDS-derived erythroid cells during ex vivo differentiation.

From: Severe ineffective erythropoiesis discriminates prognosis in myelodysplastic syndromes: analysis based on 776 patients from a single centre

Fig. 2

a Gene set enrichment analysis (GSEA) of aging and apoptotic_signaling_pathway in hematopoietic cells at various differentiation stages from MDS patients and HD, respectively. Normalized Enrichment Score (NES) and Nominal p-value are shown in each plot. b Aging Score was calculated based on FPKM of aging genes. Wilcoxon Signed-rank Test (Paired) was used to analyze the statistical significance and p values are shown in the plot. c The expression of ERCC1, one of the aging-associated genes, at day 8, 11 and 14 of erythroid differentiation in MDS patients and HD by qRT-PCR. d The expression of ERCC1 was measured by qRT-PCR and WB, respectively, in primary erythroid cells at day 8 of differentiation after lentiviral-mediated knockdown. e The representative β-galactosidase staining images of day 8-differentiated primary erythroid cells in ERCC1 diminished or scramble control cells (Scale bar = 20 μm). f The bar graph showing the percentage of β-galactosidase positive cells in control and ERCC1-diminished cells at day 8 of erythroid differentiation derived from cord blood CD34+ cells.

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