Fig. 7: Downregulation of PA28α decreases proteasome workload. | Blood Cancer Journal

Fig. 7: Downregulation of PA28α decreases proteasome workload.

From: Downregulation of PA28α induces proteasome remodeling and results in resistance to proteasome inhibitors in multiple myeloma

Fig. 7

a Western blot analysis of ubiquitinated proteins in total protein lysates extracted from LP1 and RPMI8226 PA28α knockdown stable cells. β-actin as a loading control. b OPP pulse-chase assay of proteasome degradation and protein synthesis in LP1 PA28α knockdown stable cells. Western blot analysis of immunoglobulin lambda light chain (ƛ IgL) (c), eIF2α, p62/SQSTM1, and LC3B (d) in LP1 and RPMI8226 PA28α knockdown stable cells, β-actin as a loading control. **P < 0.01, Student’s t test. e Working model of PA28α knockdown in MM.

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