Fig. 2: Transcriptional repression of RASSF2 contributes to the t(8;21)-associated gene expression signature in AML. | Blood Cancer Journal

Fig. 2: Transcriptional repression of RASSF2 contributes to the t(8;21)-associated gene expression signature in AML.

From: The RUNX1-ETO target gene RASSF2 suppresses t(8;21) AML development and regulates Rac GTPase signaling

Fig. 2

a Western blot showing RASSF2 protein in THP-1 cells following lentiviral transduction with indicated shRNAs. Data for nine independent replicates (three shCTRL, six shRASSF2) that were submitted for RNA-sequencing analysis are included. b Volcano plot of RNA-sequencing analysis depicting fold-change expression and significance for all detected genes in human THP-1 cells with stable transduction of two independent RASSF2-targeting shRNAs (shRASSF2, three replicates each) compared to control-targeting shRNA (shCTRL, three replicates). Significantly changed genes are highlighted (downregulated, blue; upregulated, red). c Gene-set enrichment plots as indicated derived from gene expression analysis in b. NES normalized enrichment score, FDR false discovery rate.

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