Fig. 4: RASSF2-mediated tumor suppression is dependent on interaction with Hippo kinases MST1/2 in t(8;21) AML.

a Schematic of RASSF2 proteins expressed in experiments b–e, RA, Ras-association domain, SARAH, Salvador-Rassf-Hippo domain. b Colony number (per 10,000 cells plated), c total viable cell number (per 10,000 cells plated), d frequency of Annexin-V+ apoptotic cells, and e representative images of colonies for RUNX1-ETO serial replating/colony formation assay with co-transduction of indicated vectors. Data are normalized relative to empty-vector control-transduced populations and presented as mean ± s.e.m. of four independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, two-tailed Student’s t-test. f RUNX1-ETO primary murine serial replating/colony formation assay was performed in mice of three indicated genotypes, with expression of RASSF2 or empty-vector control, as shown in schematic (left). Colony numbers (per 10,000 cells plated) are shown for third and fourth week of replating for each genotype (right). Data are mean ± s.e.m. of three experiments. *p < 0.05, two-tailed Student’s t-test.