Fig. 6: Nfkbie deficiency enhances GC B-cell formation. | Blood Cancer Journal

Fig. 6: Nfkbie deficiency enhances GC B-cell formation.

From: Nfkbie-deficiency leads to increased susceptibility to develop B-cell lymphoproliferative disorders in aged mice

Fig. 6

a Scheme of the immunization protocol. Mice were intraperitoneally immunized with SRBC on day 0 (D0) and analyzed 10 days afterward (D10). Representative flow cytometry analysis of splenocytes from SRBC-immunized mice of the indicated genotypes. Gates depict germinal center (GC) B cells (B220+CD19+CD95(Fas)+GL7+). Percentages and absolute cell numbers of GC B cells in the spleen of immunized mice are shown. Each symbol represents one mouse (n = 4). b Flow cytometry plots showing IgM and IgG1 expression by GC (B220+CD19+CD95(Fas)+GL7+) B cells. Histograms show percentages of IgG1+ and IgM+ Nfkbie+/+, and Nfkbie−/− GC B cells. Each symbol represents one mouse (n = 4). c Splenic plasma cells generation after SRBC immunization of Nfkbie+/+ and Nfkbie−/− mice; shown as FACS profiles (upper panel) and quantitation (lower panel). Each symbol represents one mouse (n = 4). d Memory B cells formation after SRBC immunization of Nfkbie+/+ and Nfkbie−/− mice; shown as FACS profiles (upper panel) and quantitation (lower panel). Each symbol represents one mouse (n = 4). Data are mean ± SEM. *p < 0.05, **p < 0.01; ns, not significant.

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