Fig. 1: XPO1 transcripts positively correlate to leukemic markers CD4/IL2RA/CADM1, TP53/NKFB1 transcripts and clinical progression in Adult T-cell Leukemia patients.

A XPO1 transcripts were analyzed by transcriptome-wide correlation in PBMC from healthy controls and Adult T-cell leukemia patients from the Brazilian cohort. Data are expressed as RMA (Robust Multi-array Average). Spearman correlation was used (all p < 0.05). B XPO1 levels increase with clinical progression to aggressive ATL. XPO1 transcript levels were quantified in matched samples from the Japanese cohort: healthy controls (HC), asymptomatic carriers (AC), and patients with smoldering, chronic and acute ATL (*p < 0.05 ANOVA with post-test for linear trend). Inset: XPO1 expression does not differ between Hbz-high expressing (>10 transcripts per million, TPM) or Hbz-low expressing (<10 TPM) ATL patients, or between Tax-positive and Tax-negative ATL patient samples (p > 0.7 for both). C Whole Genome Correlation Network Analysis (WGCNA) demonstrates XPO1 belongs to the same transcriptional module as leukemic marker CADM1, apoptosis regulators TP53 and caspases (CASP3-7-10). Spearman test was used for correlation with WGCNA gene module ‘eigengene’ (confirming the similarity in the XPO1 gene module in both Brazilian and Japanese cohorts) and patient data (age, survival, flow cytometry, ex vivo apoptosis measured after short-term culture of primary cells) for the Brazilian cohort (left panel) and CD4 and CD8B transcripts for the Japanese cohort (right panel).